https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20566 Wed 11 Apr 2018 10:41:17 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30059 P for heterogeneity ≥0.36), although associations were strongest in relation to the POAG subtypes with IOP <22 mmHg (MVRR, 1.93; 95% CI, 1.09–3.43) and early paracentral VF loss (MVRR, 2.27; 95% CI, 1.32–3.88). Conclusions: Although the number of natural teeth was not associated with risk of POAG, recent tooth loss was associated with an increased risk of POAG. Because these findings may be due to chance, they need confirmation in larger studies.]]> Tue 03 Oct 2017 13:26:04 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40563 Thu 14 Mar 2024 08:35:14 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1074 Sat 24 Mar 2018 08:32:11 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26685 ex-vivo. Explants were treated either with Z-VAD-FMK (a pan-caspase inhibitor; 100µM) or vehicle. Retinal Ganglion cell (RGC) density was analysed by βIII tubulin and RNA-binding protein with multiple splicing (RBPMS) immunohistochemistry. Caspase activity was measured using Caspase 3/7 glo assay and western blot. Caspase-3 expression was quantified using RT-PCR and western blotting. Retinal explants treated with Z-VAD-FMK demonstrated a 1.5-fold (p = 0.027) increase in number of surviving RGCs on day 4 compared to the control treatment using ßIII tubulin staining. RGC viability was 2-fold (p = 0.002) higher in RGC stained with RBPMS on day 1 compared to control. There was no RBPMS staining of RGCs beyond day 1 in either treatment. The caspase activity was 4.75 and 5.5-fold (p = 0.002 and 0.004 respectively) higher in control as compared to treatment with Z-VAD-FMK on day 1, 2 respectively. Increase in caspase activity in control group was also confirmed by western blot for day 1 protein lysates. Caspase-3 mRNA expression was 4.75-fold higher in Z-VAD-FMK treated explants compared to control on day 1 (p < 0.001). Culture conditions appropriate to retinal explant culture for investigation of RGC apoptosis was identified. Retinal cultures at day 4 were ideal for detecting neuroprotection using ßIII tubulin staining. RBPMS acts as a viability marker as well as to define best time point for investigation of apoptosis related signalling pathways which is day 1. These findings suggest that mouse retinal explants are good model for studying ganglion cell specific apoptosis and are applicable to diseases such as glaucoma.]]> Sat 24 Mar 2018 07:26:17 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49881 Fri 09 Jun 2023 10:36:37 AEST ]]>